E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 03 Dec 2024
 


An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals

Asmaa E. Gamal El-deen, Rania S.m., Abdelhalim A. Elshawadfy, El-gedawy A.a..


Abstract
Mycobacterium avium subspecies paratuberculosis(MAP) is one of the most infectious bacteria affecting small and large ruminants causing Johne's disease which is characterized by chronic diarrhea , severe emaciation and decreasing of the milk production leading to highly effective economic losses. Also, MAP causes Johne's disease in human. In this study, blood without anticoagulant (n=200) and faecal (n=200) samples were collected from sheep, goats, cattle and buffaloes. By using of ZN stain, smears from decontaminated faecal samples revealed presence of acid fast bacilli (68%). Serum samples separated from blood tested by ELISA showed the presence of antibodies against MAP in sheep (20%), goats (17.1%), cattle (22.5%), and buffaloes (16%). Faecal samples were tested by PCR for detection of IS900 specific gene for confirmation of MAP strains and it was detected in (26.7%) sheep, (25.7%) goats, (28.8%) cattle, and (20%) buffaloes. Also, the conventional PCR was used to differentiate between 2 types of MAP where S- type was detected in (9)sheep, (5) goats, (12) cattle, and (3) buffaloes and C- type was detected in (7) sheep, (4) goats, (11) cattle, and (2) buffaloes. This study proved that MAP is a widespread bacterium and shows the highly spreading of its strains in ruminants.

Key words: : MAP, Johne's disease, Ruminants, ELISA, PCR


 
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How to Cite this Article
Pubmed Style

El-deen AEG, S.m. R, Elshawadfy AA, A.a. E. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS. 2025; 84(0): 18-26. doi:10.5455/ajvs.226337

Web Style

El-deen AEG, S.m. R, Elshawadfy AA, A.a. E. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. https://www.alexjvs.com/?mno=226337 [Access: March 25, 2025]. doi:10.5455/ajvs.226337

AMA (American Medical Association) Style

El-deen AEG, S.m. R, Elshawadfy AA, A.a. E. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS. 2025; 84(0): 18-26. doi:10.5455/ajvs.226337


Vancouver/ICMJE Style

El-deen AEG, S.m. R, Elshawadfy AA, A.a. E. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS. (2025), [cited March 25, 2025]; 84(0): 18-26. doi:10.5455/ajvs.226337


Harvard Style

El-deen, A. E. G., S.m., . R., Elshawadfy, . A. A. & A.a., . E. (2025) An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. AJVS, 84 (0), 18-26. doi:10.5455/ajvs.226337


Turabian Style

El-deen, Asmaa E. Gamal, Rania S.m., Abdelhalim A. Elshawadfy, and El-gedawy A.a.. 2025. An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. Alexandria Journal of Veterinary Sciences, 84 (0), 18-26. doi:10.5455/ajvs.226337


Chicago Style

El-deen, Asmaa E. Gamal, Rania S.m., Abdelhalim A. Elshawadfy, and El-gedawy A.a.. "An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals." Alexandria Journal of Veterinary Sciences 84 (2025), 18-26. doi:10.5455/ajvs.226337


MLA (The Modern Language Association) Style

El-deen, Asmaa E. Gamal, Rania S.m., Abdelhalim A. Elshawadfy, and El-gedawy A.a.. "An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals." Alexandria Journal of Veterinary Sciences 84.0 (2025), 18-26. Print. doi:10.5455/ajvs.226337


APA (American Psychological Association) Style

El-deen, A. E. G., S.m., . R., Elshawadfy, . A. A. & A.a., . E. (2025) An Outlook on the Molecular Characterization of Mycobacterium Avium Subspecies Paratuberculosis Strains in Farm Animals. Alexandria Journal of Veterinary Sciences, 84 (0), 18-26. doi:10.5455/ajvs.226337




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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